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| 1. |  | DE CARVALHO NUNES, L.; STEGELMEIER, B. L.; COOK, D.; PFISTER, J. A.; GARDNER, D. R.; RIET-CORREA, F.; WEICH, K. D. Clinical and pathological comparison of Astragalus lentiginosus and Ipomoea carnea poisoning in goats. Toxicon, 5 December, 2019, Volume 171, Pages 20-28. Doi: 10.1016/j.toxicon.2019.09.016 Article history: Received 12 July 2019; Received in revised form 12 September 2019; Accepted 16 September 2019; Available online 19 September 2019.| Biblioteca(s): INIA Las Brujas. |
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 | Acceso al texto completo restringido a Biblioteca INIA La Estanzuela. Por información adicional contacte bib_le@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela. |
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Fecha actual : |
20/07/2022 |
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Actualizado : |
20/07/2022 |
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Tipo de producción científica : |
Capítulo en Libro Técnico-Científico |
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Autor : |
CRISPO, M.; CHENOUARD, V.; DOS SANTOS-NETO P; TESSON, L.; SOUZA-NEVES, M.; HESLAN, J.M.; CUADRO, F.; ANEGÓN, I.; MENCHACA, A. |
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Afiliación : |
MARTINA CRISPO, Laboratory Animal Biotechnology Unit, Institut Pasteur de Montevideo, Montevideo, Uruguay.; VANESSA CHENOUARD, INSERM Centre de Recherche en Transplantation et Immunologie UMR 1064, Transgenesis Rat ImmunoPhenomic Facility (TRIP), Nantes, France.; PEDRO DOS SANTOS-NETO, Instituto de Reproducción Animal Uruguay, Fundación IRAUy, Montevideo, Uruguay.; LAURENT TESSON, INSERM Centre de Recherche en Transplantation et Immunologie UMR 1064, Transgenesis Rat ImmunoPhenomic Facility (TRIP), Nantes, France.; MARCELA SOUZA-NEVES, Instituto de Reproducción Animal Uruguay, Fundación IRAUy, Montevideo, Uruguay.; JEAN-MARIE HESLAN, INSERM Centre de Recherche en Transplantation et Immunologie UMR 1064, Transgenesis Rat ImmunoPhenomic Facility (TRIP), Nantes, France.; FEDERICO CUADRO, Instituto de Reproducción Animal Uruguay, Fundación IRAUy, Montevideo, Uruguay.; IGNACIO ANEGÓN, INSERM Centre de Recherche en Transplantation et Immunologie UMR 1064, Transgenesis Rat ImmunoPhenomic Facility (TRIP), Nantes, France.; JOSE ALEJO MENCHACA BARBEITO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay./Instituto de Reproducción Animal Uruguay, Fundación IRAUy, Montevideo, Uruguay. |
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Título : |
Generation of a Human Deafness Sheep Model Using the CRISPR/Cas System. |
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Complemento del título : |
Chapter 12. |
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Fecha de publicación : |
2022 |
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Fuente / Imprenta : |
Methods in Molecular Biology, 2022, Volume 2495, Pages 233-258. doi: https://doi.org/10.1007/978-1-0716-2301-5_12 |
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DOI : |
10.1007/978-1-0716-2301-5_12 |
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Idioma : |
Inglés |
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Contenido : |
Abstract:
CRISPR/Cas9 system is a promising method for the generation of human disease models by genome editing in non-conventional experimental animals. Medium/large-sized animals like sheep have several advantages to study human diseases and medicine. Here, we present a protocol that describes the generation of an otoferlin edited sheep model via CRISPR-assisted single-stranded oligodinucleotide-mediated Homology-Directed Repair (HDR), through direct cytoplasmic microinjection in in vitro produced zygotes.Otoferlin is a protein expressed in the cochlear inner hair cells, with different mutations at the OTOF gene being the major cause of nonsyndromic recessive auditory neuropathy spectrum disorder in humans. By using this protocol, we reported for the first time an OTOF KI model in sheep with 17.8% edited lambs showing indel mutations, and 61.5% of them bearing knock-in mutations by HDR . The reported method establishes the bases to produce a deafness model to test novel therapies in human disorders related to OTOF mutations. |
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Palabras claves : |
Embryo; Genome editing; In vitro fertilization; OTOF; SsODNs. |
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Thesagro : |
OVEJA. |
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Asunto categoría : |
-- |
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Marc : |
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024 7 $a10.1007/978-1-0716-2301-5_12$2DOI
100 1 $aCRISPO, M.
245 $aGeneration of a Human Deafness Sheep Model Using the CRISPR/Cas System.$h[electronic resource]
260 $c2022
520 $aAbstract: CRISPR/Cas9 system is a promising method for the generation of human disease models by genome editing in non-conventional experimental animals. Medium/large-sized animals like sheep have several advantages to study human diseases and medicine. Here, we present a protocol that describes the generation of an otoferlin edited sheep model via CRISPR-assisted single-stranded oligodinucleotide-mediated Homology-Directed Repair (HDR), through direct cytoplasmic microinjection in in vitro produced zygotes.Otoferlin is a protein expressed in the cochlear inner hair cells, with different mutations at the OTOF gene being the major cause of nonsyndromic recessive auditory neuropathy spectrum disorder in humans. By using this protocol, we reported for the first time an OTOF KI model in sheep with 17.8% edited lambs showing indel mutations, and 61.5% of them bearing knock-in mutations by HDR . The reported method establishes the bases to produce a deafness model to test novel therapies in human disorders related to OTOF mutations.
650 $aOVEJA
653 $aEmbryo
653 $aGenome editing
653 $aIn vitro fertilization
653 $aOTOF
653 $aSsODNs
700 1 $aCHENOUARD, V.
700 1 $aDOS SANTOS-NETO P
700 1 $aTESSON, L.
700 1 $aSOUZA-NEVES, M.
700 1 $aHESLAN, J.M.
700 1 $aCUADRO, F.
700 1 $aANEGÓN, I.
700 1 $aMENCHACA, A.
773 $tMethods in Molecular Biology, 2022, Volume 2495, Pages 233-258. doi: https://doi.org/10.1007/978-1-0716-2301-5_12
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